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ZERO
5
1
3
4.0ml
Sample
2
4
15 min
PROCEDURE for Free Iron
applies to DR/4000, DR/2500 DR/2010 and DR/800
Note
If this is the first time you have run this revision of the test on
your DR/4000, you must contact your local Hach distributor
for an upgrade of your software (Cat.No. 49544-00). Software
Version number 2.39 contains the factory UniCell™ programs.
INSTRUMENT SET-UP
1. Press the soft key under HACH PROGRAM (or
USER PROGRAM if the method was entered as a
USER PROGRAM).
Enter program number 2130 (or 159 if the method was
entered as a USER PROGRAM).
Press ENTER.
2. The display will show:
HACH PROGRAM: 2130
Iron, HCT 159
The wavelength (
λ), 485 nm, is automatically selected.
3. Insert the Test N’Tube adapter into the cell holder by
sliding it under the thumb screw and into the alignment
grooves. Fasten in place by tightening the thumb screw.
4. Follow the procedure steps.
(Note: If measuring Total Iron and performed the sample
digestion using the HCT 200 Metal Prep Set, select the
Total Iron alternate form under the Options soft key menu).
5. Wipe the outside of the zero vial.
Place the zero vial into the adapter.
Close the light shield.
6. Press the soft key under ZERO.
The display will show: 0.00 mg/L Free-Fe or
0.00 mg/L Tot-Fe if the Total Iron alternate form
was selected.
7. Clean the outside of the sample vial.
8. Place the prepared sample vial into the adapter. Close
the light shield. Results in mg/L Free-Fe or Tot- Fe are
displayed.
Note
If the test overranges, repeat the UniCell™ test with diluted
sample.
2.Pipet 4.0 ml sample
into the sample vial.
3. Close sample vial and
invert a few times, until
the freeze-dried
contents are
completely dissolved.
4. Start the instrument
timer.
A 15-minute reaction
period will beginn.
5. Zero the instrument
using the zero vial
(white cap) provided
and read the sample.
DR / 4000—Rev 2.39
1. Enter program number
and set up the
instrument as explained
for each instrument
type.
HCT 159 HCT 159
LIST OF REQUIRED REAGENTS
Iron-Fe
UniCell™ HCT 159 23/pkg HCT 159
OPTIONAL REAGENTS
Metal Prep Set
UniCell™ HCT 200 50 digestions HCT 200
Iron Standard
1000 mg/L as Fe 100 ml 2271-42
Sodium Hydroxide
Standard Solution 1000 ml 2450-53
(5.0 N)
Nitric Acid, concentrated 500 ml 152-49
OPTIONAL APPARATUS
Cylinder, mixing – 100 ml each 20886-42
Flask, volumetric 100 ml each 547-42
Pipettor, (Jencons) 1-5 ml each 27951-00
Replacement tips for 27951-00 pk/100 27952-00
Pipettor, (Jencons) 100-1000 µl each 27949-00
Replacement tips for 27949-00 pk/400 27950-00
pH Paper pk/100 26013-00
MEASURING RANGE:
0.10 - 5.00 mg/L Free-Fe / 0.12 - 6.00 mg/L Tot-Fe
PROCEDURE
for Total Iron:
After digestion with the Metal Prep Set HCT 200,
follow the procedure steps 1 – 5.